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. 2016 Oct 12;291(48):24951–24960. doi: 10.1074/jbc.M116.754705

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Multi-dimensional MS analysis of maltoheptaose (DP7) and maltooctaose (DP8) synthesized from unlabeled maltohexaose (DP6) using ZmSSIIa and ADP-[¹³C_U]glucose. A, the analytical scheme applied herein. Following conversion, the reducing end is modified through reductive amination. B, ESI-MS spectrum of the reaction products showing conversion to DP7 and DP8; monoisotopic masses are provided. C–E, MS-MS spectra of the parent ions for DP6 (C), DP7 (D), and DP8 (E). Ions are identified at the bottom of the figure with connections through vertical dashed gray lines and clearly indicate only modification of the DP6 non-reducing terminus.