Skip to main content
. 2016 Oct 11;291(48):24961–24973. doi: 10.1074/jbc.M116.755991

FIGURE 7.

FIGURE 7.

RPA is required to recruit Cdc24 and Dna2 to DNA break sites. A, the intensity and number of RPA foci decrease in the rpa1ts cells. The RPA1-YFP gene was integrated into its original genomic locus under the control of the native promoter. The cultures of wt and rpa1ts cells were first grown to an A590 of ∼0.3 at 26 °C and then shifted to 36.5 °C for an additional 6-h incubation. The cells were fixed and subjected to fluorescent analysis. B, Cdc24 and Dna2 are not localized to DNA break sites in the rpa1ts cells. The assays were conducted as described in Fig. 6A. The foci of Cdc24-YFP, mCherry-Dna2, and Rad52-CFP are presented. C–E, quantification of RPA (C), Cdc24 (D), and Dna2 (E) foci in the wt and rpa1ts cells. The percentage of cells having RPA, Cdc24, or Dna2 foci is arbitrarily assumed to be 100% in the wt cells.