FIGURE 1.

Increased expression of the genes for cholesterol synthesis, uptake, and transport in SH-SY5Y cells exposed to l-Nor. A, pathway of cholesterol biosynthesis. B and C, increased expression of the genes for cholesterol biosynthesis (B) as well as uptake and transport (C). The cells were treated with 3 mm l-Nor for 2, 4, or 6 h, and the relative expression levels of the genes to that of β-actin were evaluated by qPCR. Data are shown as mean ± S.D. (n = 3). Essentially the same results were obtained in two independent experiments and a typical result is shown. *, p < 0.05 versus 0 h. NPC2, Niemann-Pick disease type C2.