FIGURE 2.

Activation of SREBP-2 in SH-SY5Y cells exposed with l-Nor. A, processing of SREBP-2 into its active form in l-Nor-treated SH-SY5Y cells. The cells were treated with 3 mm l-Nor for the indicated time periods and the cell lysates were subjected to immunoblot analysis using anti-SREBP-2 antibody. Processing of SREBP-2 from its inactive precursor form (intact) to truncated active form (truncated) is shown. Actin served as a loading control. Typical result from four independent experiments is shown. B, increased levels of truncated active SREBP-2 relative to actin in l-Nor-treated SH-SY5Y cells. Data are shown as mean ± S.D. from four experiments (n = 4). *, p < 0.05 versus 0 h. C, nuclear translocation of SREBP-2 in response to l-Nor stimulation. The cells were treated with 3 mm l-Nor for 0–6 h and subjected to immunofluorescence analysis with anti-SREBP-2 antibody and Alexa 549-labeled secondary antibody (red). Nuclei were also counterstained with DAPI (blue). Typical result from two experiments is shown. Right panels show fluorescence intensity plots along the dotted lines shown in fluorescence images. Scale bar, 10 μm.