FIGURE 4.

Radioactive lipids formed by microsomes from the indicated strains. Microsomes were prepared from the indicated yeast strains bearing EV or expressing GPC1 under the GAL1 promoter (GPC1). The WT yeast was grown in YPD, and yeast strains carrying a plasmid were grown in YNB medium containing galactose. Microsomes (2 μg of protein) were incubated with [¹⁴C]choline-labeled GPC (0.24 mm) and 18:1-CoA (0.2 mm) for 30 min at 30 °C. The results shown are from triplicate assays ± S.D. (error bars).