FIGURE 5.

Biochemical characterization of Gpc1p. A, dependence of Gpc1p activity on GPC concentration in the presence of 18:1-CoA. B, the acyl-specificity of Gpc1p at optimal GPC concentration (4 mm). The activity was measured as incorporation of ¹⁴C activity from [¹⁴C]choline-labeled GPC into chloroform-soluble lipids in the presence of acyl-CoA (0.2 mm). Microsomal preparations (2 μg of protein) prepared from ale1Δ gpc1Δ expressing GAL1-GPC1 were incubated for 4 min at 30 °C. ric, ricinoleoyl-CoA. Data are given for triplicate assays ± S.D. (error bars).