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. 2016 Oct 7;291(48):25066–25076. doi: 10.1074/jbc.M116.743062

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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FIGURE 8. — Acylation of glycerophosphoethanolamine. Shown is incorporation of ¹⁴C activity from [¹⁴C]choline-labeled GPC (0.5 mm) or [¹⁴C]ethanolamine-labeled GPE (0.5 mm) into chloroform-soluble lipids in the presence of 18:1-CoA (0.4 mm). Shown are microsomal preparations (40 μg of protein) of ale1Δ gpc1Δ yeast cells with overexpressed GPC1 or EV. The radioactivity resided in LPC in assays with added [¹⁴C]GPC and in lysophosphatidylethanolamine in the case of added [¹⁴C]GPE. Incubation time was 20 min, and the temperature was 30 °C. Data are given for triplicate assays ± S.D. (error bars).