Neuropilin-2 affects cell positioning of adult-born neurons in the dentate gyrus.
A, diagram showing the granule cell layer (#1–3) and the molecular layer (#4) in adult dentate gyrus. B, representative images of shCTR or shNRP2-expressing newborn neurons in the DG at 28 DPI (scale bar, 20 μm). C, quantification of the positioning of adult-born neurons infected with retrovirus of control, shNRP1, or shNRP2. Colored bars represent the percentages of cells of each experimental group in layers 1, 2, and 3 of the granular layer and layer 4 (molecular layer). **, p < 0.01, ***, p < 0.001, as compared with shCTR, one-way ANOVA with Newman-Keuls' post hoc test.