TABLE 4.
Inhibition of c-MET and EGFR phosphorylation by EGFR × c-MET bsAbs
IC50 values were calculated upon 3PL fitting of dose-response curves using GraphPad Prism. S.D. values were calculated for at least two independent experiments carried out in duplicates. NF, the fit returned by Prism is poor or ambiguous. n = number of independent experiments.
| Antibody | Receptor | A549 |
NHEK.f-c. |
||
|---|---|---|---|---|---|
| IC50 ± S.D. | n | IC50 ± S.D. | n | ||
| nm | nm | ||||
| CS06 × 225-H | Phospho-c-MET | 0.3 ± 0.2 | 4 | 0.1 | 1 |
| Phospho-EGFR | 0.8 | 1 | 0.8 | 1 | |
| B10v5 × 225-H | Phospho-c-MET | 0.3 ± 0.2 | 2 | 0.2 | 1 |
| Phospho-EGFR | 1.1 ± 0.4 | 2 | 1.7 ± 0.3 | 2 | |
| B10v5 × 225-M | Phospho-c-MET | 0.3 ± 0.2 | 2 | 0.14 | 1 |
| Phospho-EGFR | NF | 3 | NF | 2 | |
| oa 5D5 | Phospho-c-MET | 0.8 ± 0.5 | 4 | 0.7 | 1 |
| Cetuximab | Phospho-EGFR | 0.4 ± 0.1 | 3 | 0.3 ± 0.2 | 2 |