FIGURE 1.

CRBN is conserved across the animal kingdom. A, phylogenetic tree of CRBN homologs. Depicted values indicate posterior probabilities. The scale bar references branch lengths. B, schemes representing the domain structure of human CRBN and its murine and Drosophila homologs (the structure of human CRBN is based on a previous study (64) and structures of murine and Drosophila homologs are based on those described in the Universal Protein Resource database (entries Q8C7D2 and Q9VH36, respectively). The two evolutionary conserved domains, LON domain and TBD, are indicated as filled boxes (blue and gray, respectively). Percentages of identity at the amino acid level are noted. Two immunogens used to generate OHGT-specific antibodies (rabbit anti-OHGT(1–187) and guinea pig anti-OHGT(15–33)) are represented. KLH, keyhole limpet hemocyanin.