Skip to main content
. 2016 Oct 4;291(48):25120–25132. doi: 10.1074/jbc.M116.757823

FIGURE 6.

FIGURE 6.

The ohgt mutation and ohgt knockdown in the fat body are associated with insulin signaling up-regulation and altered expression of fat body-derived cofactors of DILPs. A, the expression level of phospho-dAkt (p-dAkt) was increased in ohgtEx2/Ex2 compared with w*. The experiment was performed in triplicate. B, transcription of the genes d4EBP, dInR, and Lip3 (included as a starvation marker) in ohgtEx2/Ex2 and w* (n = 4). C, the expression level of phospho-dAkt was increased in Cg>ohgtRNAiv40486 compared with Cg>w* early third instar larval carcasses. The experiment was performed in triplicate. D, transcription of d4EBP, dInR, and Lip3 in Cg>ohgtRNAiv40486 and Cg>w* in early third instar larval carcasses (n = 4). E, transcription of the genes dALS, Imp-L2, and upd2 in the total ohgtEx2/Ex2 and w* early third instar larvae was quantified (n = 4). F, transcription of dALS, Imp-L2, and upd2 in the fat bodies of Cg>ohgtRNAiv40486 and Cg>w* early third instar larvae was quantified (n = 4). Error bars indicate S.E.