FIGURE 5.

In vitro conversion of PE to PG by ClsB. [³²P]PG was formed by mixing membranes derived from BKT29 cells expressing wild-type ClsB and partially purified ³²P-labeled PE in the presence of 100 mm glycerol. In contrast, no PG was formed using membranes from cells transformed with an empty vector or expressing the ClsB^H113A mutant. The presence of ³²P-labeled lyso-PE likely resulted from partial hydrolysis of PE. The spot at the top of the second lane concurrent with PG formation is likely acyl-PG, a known PG derivative in E. coli.