FIGURE 8.

Expression of ClsB complements the defects of a ΔpgsA::Kan^R mutant in cell growth and in PG and CL synthesis. A, a spot assay was conducted with a 10-fold series dilution of E. coli ΔpgsA::Kan^R strains covered by plasmids expressing either pgsA or clsB. The strains were grown on LB plates in the presence or absence of 0.4% glycerol and/or 0.02% arabinose. The growth of the pBAD-clsB ΔpgsA::Kan^R strain required both arabinose induction and glycerol supplementation. In contrast, the pBAD-pgsA ΔpgsA::Kan^R strain required neither. B, TLC analysis of lipid extracts from wild-type, and pBAD-pgsA and pBAD-clsB ΔpgsA::Kan^R E. coli strains.