FIGURE 8.

Knockdown of PHLPP alters the formation of aPKC-Par3 complex. A, cell lysates prepared from sh-Con, sh-PHLPP1, and sh-PHLPP2 Caco2 cells were immunoprecipitated with protein A/G beads alone (Mock) or with antibodies against PKCι and PKCζ. The presence of Par3, as well as PKCι and PKCζ, in the cell lysate input and immunoprecipitates (IP) was detected using the Par3, PKCι, and PKCζ antibodies, respectively. B, the expression of PHLPP and phosphorylation status of Akt, PKCι, and PKCζ in the cell lysates was analyzed using Western blotting.