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QCM binding analyses of GroEL G192X mutants to immobilized GroES ESC7. Preparations of ESC7 were immobilized to the sensor chip using standard methods, and aliquots of GroEL G192X were added to the cell. Signal changes reflect changes to the mass that is bound to the sensor chip, which in this case represents the amount of GroEL G192X that is bound to ESC7. With the exception of GroEL WT, all measurements were performed in the absence of ATP.
