Table 1.
Crystal Violet staining procedure (Fraser FJ method modified).21
| Xylene | 3 changes 15 min each |
| Alcohol | Hydrate through graded alcohols (100%, 90% and 80%) |
| Water | Wash in water |
| Hydrolyze | 1 N HCl at 56–60 °C for 12 min |
| Distilled water | Wash in three changes of distilled water |
| Alkaline water | Wash in two changes of alkaline water |
| 1% aqueous Crystal Violet | Stain for 30 min in Crystal Violet |
| 1% acid alcohol | Dip for 5–10 s to differentiate |
| Alcohol | Dehydrate quickly through graded alcohols (80%, 90% and 100%) |
| Xylene | Clearing for 10–15 min |
| DPX | Mounting |