Figure 1.

Evaluation of measured output ratio of spiked Arabidopsis in vitro transcript at known input ratios. A. Total thymus RNA extracted from the DR+/+ and DRlyp/lyp [6] BioBreeding rats spiked with known input ratios of Arabidopsis gene in vitro transcript and hybridized to 18,000 probe rat cDNA arrays possessing serially diluted fluorescein-labeled Arabidopsis probes. B. Evaluation of data compression as a function of support-bound probe. On the x-axis is plotted the average pixel fluorescein intensity per spot plotted against the Arabidopsis transcript measured output log ratio/actual input log ratio. As spot intensities fall below 5000 RFU/pixel, ratio measurements become increasingly compressed.