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Western blot analysis for evaluation of DT386 expression. (A) lane 1: protein marker, lane 2: un-induced E. coli BL21 (DE3) containing pET28a-DT386, lane 3: induced E.coli BL21 (DE3) containing pET28a-DT386, lane 4: uninduced E. coli BL21 (DE3) containing pET28a, lane 5: induced E.coli BL21 (DE3) containing pET28a, lane 6: uninduced E. coli BL21 (DE3), lane 7: induced E. coli BL21 (DE3). (B) lane 1: protein marker, lanes 2 to 5: elutes 1 to 4 of purified DT386.
