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. 2016 Nov 25;6:37947. doi: 10.1038/srep37947

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a–c) Overview of simulation results: Jaccard index, root-mean-square error, and Fourier ring correlation of emitters localized with different softwares at various average signal-to-noise ratio (SNR) levels. TrackNTrace is evaluated using both wavelet filtering and cross-correlation for emitter candidate detection. (d) Execution time of programs on Siemens star and high-SNR emitter grid data. (e) dSTORM imaging of a rat hippocampal neuron axon initial segment shows periodicity of βIV-spectrin in the cytoskeleton labeled with Alexa647. Inset: Normalized 1D intensity projection along the rectangle’s wide axis. (f) Example image of Atto655-labeled DPPE diffusing in a BLM. (g) Diffusion coefficients obtained for lipid bilayer experiments. Scale bar, 1 μm (e,f).