Table 4. Regional mRNA expression of melanocortin receptors 3 (MC3R) and 5 (MC5R), percent expression relative to Mc4r+/+ (100%); circulating and adipose cytokines, Mean (SEM).
| Genotype | ARC |
PVN |
PeFLH |
VMH |
DMH |
Cytokine (pg/ml) |
WAT | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MC3R | MC5R | MC3R | MC5R | BDNF | MC3R | MC5R | MC3R | MC5R | BDNF | MC3R | MC5R | Leptin | IL-1α | IL-1β | IL-6 | IL-10 | TNF-α | TNF-α | |
| Mc4rK314X/K314X | 158 (32) | 101 (7) | 109*,† (2) | 108 (3) | 102† (3) | 104 (2) | 102 (5) | 149 (8) | 97 (7) | 120 (9) | 129 (11) | 125 (14) | 11962.00*,† (808.26) | 44.58 (6.65) | 387.85 (89.10) | 33.76 (2.14) | 293.89 (84.58) | 25.79 (2.90) | 349 (29) |
| Mc4r+/K314X | 112 (4) | 98 (9) | 96 (3) | 94 (4) | 91* (3) | 156 (7) | 115 (7) | 131 (14) | 96 (3) | 108 (7) | 122 (14) | 132 (12) | 9843.50 (541.98) | 37.31 (2.00) | 205.99 (34.16) | 35.36 (2.82) | 170.06 (30.88) | 23.14 (0.64) | 383 (48) |
| Mc4r+/+ | 100 (3) | 100 (6) | 100 (4) | 100 (4) | 100 (1) | 100 (6) | 100 (1) | 100 (12) | 100 (10) | 100 (7) | 100 (5) | 100 (5) | 9425.75 (534.51) | 43.40 (6.30) | 370.06 (92.45) | 34.73 (3.33) | 257.50 (66.48) | 26.06 (1.75) | 537 (195) |
Rats homozygous (Mc4rK314X/K314X, n = 5) or heterozygous (Mc4r+/K314X, n = 6) for non-functional melanocortin 4 receptor (MC4R), or wild-type rats (Mc4r+/+, n = 4).
ARC: arcuate nucleus; PVN: paraventricular nucleus; PeFLH: perifornical lateral hypothalamus; VMH: ventromedial hypothalamus; DMH: dorsomedial hypothalamus; WAT: epididymal white adipose tissue. Cytokines (n = 8/group) interleukins (IL) 1α, 1β, 6, and 10, and tumor necrosis factor- α (TNF- α).
*significantly different from Mc4r+/+; †significantly different from Mc4r+/K314X; p < 0.05.