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. 2016 Nov 25;6:37684. doi: 10.1038/srep37684

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative lung sections of animals with and without flow-induced pulmonary hypertension (A). Morphometric analysis of the medial thickness of the intralobular (ϕ 500–1000 μm, (B) and intraacinar (ϕ <250 μm, C) pulmonary artery. Thickness of medial layer is expressed as wall thickness to lumen radius (WT/LR) ratio (%). Thickness of medial layer was significantly reduced 4 weeks (lig 4w) after ligation of aortocaval fistula. *P < 0.05 compared with sham and ^†P < 0.05 compared with lig 4w. Magnifications are 100× for the upper panel and 200× for the lower panel. Analysis was performed in 5–6 different lung sections in each group, and the medical thickness was measured in at least 1 intralobular and 2 intraacinar pulmonary artery in each tissue section using the ImageJ software. Data were analyzed by one-way ANOVA and are shown as mean ± SD.