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. 2016 Nov 25;6:37642. doi: 10.1038/srep37642

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Copyright © 2016, The Author(s)

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Both serovars were cultured in N-Salt medium with 8 μM, 1 mM, and 10 mM Mg²⁺ concentrations. Then the total RNA were isolated and analyzed by qPCR. The expression level of hilD (A), and pagC (B) in both serovars was examined in different Mg²⁺ concentrations. All the expression levels are relative to the 16S rDNA. *p < 0.05.