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. 2016 Nov 25;6:37827. doi: 10.1038/srep37827

Figure 6. CXCR4−/− HSPCs exhibit enhanced sensitivity to oxidative stress.

Figure 6

Donor-derived BM cells from CXCR4+/+ and CXCR4−/− chimeras 6 weeks post transplantation were sorted and cultured for 24 hr in the presence of a low BSO concentration (10 μM), with or without CXCL12. (a) Colony formation. Mean ± SEM of 3 independent experiments performed in duplicate. (b–d) DCF MFIs on CXCR4−/− BM LSK-SLAM (b), LK (c) and BM-MNC cells (d). (e–g) DCF MFIs on CXCR4+/+ BM LSK-SLAM (e), LK (f) and BM-MNC cells (g). Mean ± SEM of 3 independent experiments, with 3 mice per group. (h) GSH/GSSG ratios in sorted LSK and LK cells from CXCR4+/+ and CXCR4−/− chimeras, 6 weeks after transplantation. Mean ± SEM of 3 independent experiments performed in triplicate, with 4 mice per group. (i,j) CFC frequencies in sorted CD45.2+ BM and in peripheral blood cells from CXCR4+/+ and CXCR4−/− chimeras determined 3 months after transplantation. Sorted cells were plated with vehicle, NAC (2 mM) or p38 MAPK inhibitor SB203580 (25 μM). Mean ± SEM of 3 independent experiments performed in duplicate.