Figure 4. Propensity for oligomerization of wild-type α-Syn and α-Syn(A53T) especially incorporated from outside the cells.

SH-SY5Y cells were cultured with the vehicle (none), 1 μM wild-type α-Syn or 1 μM α-Syn(A53T) for various time periods as indicated. In some experiments cells were transfected with empty vector (pCMV5), vector encoding wild-type α-Syn, or α-Syn(A53T) and cultured for 48 hr. Cells were then lysed and subjected to immunoblotting with anti-α-Syn antibody. The results are the representative of 3 independent experiments.