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. 2016 Nov 25;6:37810. doi: 10.1038/srep37810

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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SH-SY5Y cells, which had been cultured in the absence of serum for 18 hr without or with 1 μM α-Syn(A53T), were stimulated for 8 min without (none) or with 20 ng/ml PDGF. After fixation, actin filaments were stained by rhodamine-phalloidin. The results are the representative of 3 independent experiments. Arrows show PDGF-induced lamellipodia formation. Note that PDGF causes filopodia (see arrow heads) instead of lamellipodia in α-Syn(A53T)–treated cells. Bars, 10 μm.