Table 1. Standard deviation in measured FRET ratio for different MDS values and glucose concentration in culture medium for: all spheroids, intra-plate and inter-plate.

MDS (μm)	Glucose concentration in culture medium (mM)	Standard deviation in FRET ratio
		Across all spheroids	Intra-plate	Inter-plate
0–10	0	0.12	0.07	0.09
	25	0.25	0.21	0.16
60–70	0	0.13	0.08	0.1
	25	0.75	0.55	0.61