Figure 1. Glutamate immunoreactivity in the spinal cord at the level of the 5th gill in a control un-lesioned sea lamprey.
(A) Confocal photomicrograph of a transverse section of the spinal cord showing the 3 regions in which the white matter was divided to quantify the number of glutamate immunoreactive (-ir) profiles. Dorsomedial (DM), ventromedial (VM) and lateral (LAT). DC indicates the dorsal column. (B) Schematic drawing of a transverse section of the spinal cord showing the glutamate-ir cell types in the larval spinal cord. (C–G) High magnification photomicrographs showing details of glutamate-ir cells (green) and nuclear counterstain (blue). (C) Cells of the dorsal population. (D) Cells of the lateral population. (E) Cerebrospinal fluid-contacting (CSFc) cells. (F) Edge cells. (G) Cells associated to the Mauthner axon. Arrows point to glutamate-ir cells. Asterisks point to Mauthner axon. Star indicates the central canal. In all photomicrographs, dorsal is at the top and the midline to the right, except for (F), in which the midline is on the left.