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. 2016 Nov 25;6:37800. doi: 10.1038/srep37800

Figure 3. Comparison of the polymerase activity in 293-T cells.

Figure 3

293-T cells were transfected with the plasmids pHW2000-PB1, -PB2, -NP and -PA (with and without the substitutions), together with a plasmid encoding firefly luciferase reporter flanked by IAV untranslated region as well as a plasmid encoding eGFP as an internal control. Luciferase activity was determined at 24 hours post transfection. Experiments were independently performed three times. Data presented are the relative polymerase activity of the indicated mutants to that of WT-H7N7 (means ± SD).