Figure 1. Structural transitions in PEP-19 on binding to apo C-CaM are restricted to its acidic sequence and the core IQ motif.

(a) Backbone amide chemical shift changes calculated using equation (1) in ¹⁵N-labelled PEP-19 on binding to apo C-CaM. (b) Steady-state ¹⁵N{¹H} NOEs of ¹⁵N free PEP-19 (open circles) and ¹⁵N PEP-19 bound to apo C-CaM (closed circles). The ¹⁵N{¹H} NOE values and the uncertainty of NOE values (error bars) were determined as described in the ‘Methods' section.