Table 1. Experimental restraints and structural statistics for an ensemble of 20 NMR structures for the PEP-19:apo C-CaM complex.

NMR distance, RDC and dihedral constraints
Distance restraints
Total NOEs	1,926
C-CaM
Intraresidue (|i−j|=0)	599
Sequential (|i−j|=1)	280
Medium range (1<|i−j|<5)	213
Long range (|i−j|≥5)	174
PEP-19 (Phe30–Ser62)
Intraresidue (|i−j|=0)	212
Sequential (|i−j|=1)	120
Medium range (1<|i−j|<5)	113
Long range (|i−j|≥5)	1
Intermolecular	198
Ambiguous	16
Total predicted hydrogen bonds	98
C-CaM	62
PEP-19 (Phe30–Ser62)	36
Total RDC restraints (H–N)	60
C-CaM	60
PEP-19	0
Total dihedral angle restraints	162
C-CaM	112
PEP-19 (Phe30–Ser62)	50
Structural statistics
Violations
Distance constraints (Å)	0.029±0.002
Dihedral angle constraints (°)	0.34±0.08
RDC constraints (Hz)	0.096±0.013
Max. distance constraint violation (Å)	<0.5
Max. dihedral constraint violation (°)	<5.0
Deviations from idealized geometry
Bond lengths (Å)	0.002±0.000
Bond angles (°)	0.496±0.005
Impropers (°)	0.429±0.016
Average pairwise RMSD (Å)
C-CaM (Glu81–Ala128 and Gln135–Thr146)
Backbone	0.40±0.08
All heavy atoms	1.32±0.10
PEP-19 (Phe30–Lys57)
Backbone	0.53±0.12
All heavy atoms	1.53±0.15

RDC, residual dipolar couplings; RMSD, root-mean-square deviation.