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. 2016 Nov 24;7:13595. doi: 10.1038/ncomms13595

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Copyright © 2016, The Author(s)

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(a) Sequence of the SSV1 T6 promoter used in PIC formation. The BRE/TATA motif and the +1 position are highlighted in bold and the non-complementary −4 to −1 regions are shown in red. (b) EMSA monitoring the formation of the archaeal PICs. Minimal archaeal PICs contain promoter DNA, TBP, TFB and RNAP. The addition of ORF145/RIP inhibits the formation of PICs in a dose–response dependent manner. The inclusion of TFEαβ leads to a supershift of the minimal PIC, which remains sensitive to ORF145/RIP. In the left panel RNAP and ORF145/RIP were incubated prior to the addition to DNA–TBP–TFB, while in the right panel PICs were allowed to form prior to the addition of ORF145/RIP.