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. 2016 Nov 24;7:13595. doi: 10.1038/ncomms13595

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Copyright © 2016, The Author(s)

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(a) Homology model of ORF145/RIP prepared with I-TASSER (C-score −2.12, TM-score 0.46±0.15) shown as a cartoon representation with the C-terminal tail (114–145) coloured red. (b) CD spectrum of ORF145/RIP (red trace) confirms a very high alpha-helical content of the recombinant protein when compared with the spectra of proteins with known structures in Dichroweb (blue circles). (c) Truncation of the small C-terminal tail (Δ114–145) abrogates the binding of ORF145/RIP to RNAP. The EMSA shows that unlabelled wild-type ORF145/RIP competes for ³²P-ORF145/RIP binding to RNAP, while ORF145^Δ114–145 does not. (d) ORF145^Δ114–145 does not inhibit abortive transcription. Error bars represent standard deviation of at least eight separate spectra.