Skip to main content
. 2016 Nov 24;7:13586. doi: 10.1038/ncomms13586

Figure 5. GM130 interacts with VPS15 but not VPS34.

Figure 5

(a) Control and patient fibroblasts were grown in complete medium (+FCS) or deprived of serum (−FCS) for 24 h. Immunoprecipitations with VPS15 or VPS34 antibodies were done on cell lysates and GM130 was detected by western blot. GAPDH was used as a loading control. (b) Control (ctrl) and patient (II.5) fibroblasts were grown and deprived of serum for 24 h. Immunofluorescence against VPS15 (red) and GM130 (green) and DAPI staining (blue) was performed and the cells were observed with a confocal microscope. (c) Control (ctrl) and patient (II.5) fibroblasts were grown in complete medium (+FCS) or deprived of serum (−FCS) for 24 h. Immunofluorescence against VPS15 (green) and GMAP210 (red) and DAPI staining (blue) was performed and the cells were observed with a confocal microscope.