Skip to main content
. 2016 Nov 24;7:13656. doi: 10.1038/ncomms13656

Figure 3. Physical interaction between SNP rs4487645 and CDCA7L promoter and the regulation of IRF4 on CDCA7L.

Figure 3

Long-range interactions detected between fragments mapping to rs4487645 and the CDCA7L promoter in GM11992 (a) and KMS11 (b). Relative interaction frequencies between rs4487645 and target regions ∼70 kb upstream were determined by 3C-qPCR, comparing the relative abundance of ligation products formed between the fragment mapping to rs4487645 and each of the target fragments±s.e.m., normalized to the relative abundance of intersite control region. Each qPCR reaction was performed with three technical replicates. The assay was performed independently for three times in both GM11992 and KMS11. (c) qPCR analysis of the siRNA knockdown of IRF4. Data shown are the mean IRF4 and CDCA7L mRNA levels±s.e.m. relative to the GAPDH reference mRNA level, normalized to control siRNA (NC siRNA). Data also show relative mRNA level of IRF4 and CDCA7L for mock transfection without siRNA oligos. Each qPCR reaction was performed with three technical replicates. P values were determined with two-tailed t-test over three biological replicates.