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. 2016 Nov 24;7:13517. doi: 10.1038/ncomms13517

Figure 4. Robo4ΔCD inhibits dermal vascular permeability and wound healing.

Figure 4

(a) Miles dermal permeability analysis. Evans blue leakage was assessed 30 min after intradermal injection of saline or 50 ng VEGF into the indicated mice. Animals were PBS perfused before imaging and quantification. Note increased leak in VEGF treated Robo4−/−;Stg compared with Robo4+/+ and Robo4−/−;Robo4ΔCD mice. Scale bar, 2 mm. (b) Quantification of Evans blue dye in the skin. N= 5–8 mice per group. Error bars represent s.e.m. *P<0.05, Mann-Whitney U test. (c,d) Representative images of wound healing of mice with the indicated genotypes at day 0 and 7 after cutaneous punch biopsy. Scale bar, 1 mm. (e) Quantification of percentage of wound area at day 7 after wounding. N=14 wounds (7 mice) in each group. Error bars: s.e.m. *P<0.05; **P<0.01; ***P<0.001, Mann–Whitney U test.