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. 2016 Oct 13;5(12):1162–1174. doi: 10.1016/j.molmet.2016.10.001

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure S2 — Dependency of daily energy expenditure on body composition. Correlation between daily energy expenditure (HP_{22 °C, ad-lib.}) and lean mass (A) or fat mass (B) following 4 wks of feeding CD, PHFD, or LHFD. SPF CD: n = 9; SPF PHFD: n = 10; SPF LHFD: n = 10; GF CD: n = 8; GF PHFD: n = 9; GF LHFD: n = 10. Linear regression of GF or SPF C57BL/6N mice was calculated irrespective of diet and microbiota status due to small sample size. (A) r² = 0.26, p < 0.001; (B) r² = 0.14, p < 0.01. There was no further dependency between daily energy expenditure and lean mass (C) or fat mass (D) after adjusting daily energy expenditure for body composition (HP_{adj.,22 °C, ad-lib.}) by ANCOVA. (C) r² < 0.001, p = ns; (D) r² < 0.001, p = ns. adj.: adjusted; ad-lib.: ad-libitum; HP: heat production.