Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 13;5(12):1162–1174. doi: 10.1016/j.molmet.2016.10.001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Specific dominant gut bacteria are affected by dietary fat source. Metagenomic DNA isolated from fecal samples (n = 24) was used for amplification of the V3/V4 region of 16S rRNA genes and subsequent sequencing using the Illumina technology. Sequences were analyzed using in-house developed pipelines as described in detail in the methods section. (A) Alpha-diversity analysis. (B) Multidimensional scaling showing differences in diversity between samples (beta-diversity) based on general UniFrac distances. (C) Box plots showing relative sequence abundance of taxonomic groups that were significantly different between mice fed the CD or HFD. (D) Phylotype analysis shown as heatmap of OTU abundances which were significantly different between the two HFD. The identity of OTUs was obtained using EzTaxon based on sequences of approximately 380 bp [64]. Best hits are shown with corresponding sequences similarity. (E) Pearson correlation analysis of Acetatifactor sp. against cecal bile acid concentrations.