Table 1.

ANCOVA for the effects of fetal age, gender, and PEMCS for DNA methylation levels in the first trimester placentas, fetal livers, and small intestines

Tissue	Assay	Variable	F value	P value
Placenta	5-mC ELISA	Age	8.567	0.008*
		Gender	8.434	0.008*
		PEMCS	0.704	0.411
	AluYb8	Age	0.059	0.811
		Gender	4.527	0.042*
		PEMCS	2.958	0.096
	LINE-1	Age	0.376	0.544
		Gender	0.013	0.909
		PEMCS	1.017	0.320
Liver	5-mC ELISA	Age	1.214	0.286
		Gender	0.191	0.667
		PEMCS	0.001	0.977
	AluYb8	Age	3.190	0.085
		Gender	0.004	0.953
		PEMCS	0.106	0.747
	LINE-1	Age	0.263	0.612
		Gender	8.779	0.006*
		PEMCS	0.672	0.419
Small intestine	5-mC ELISA	Age	3.576	0.078
		Gender	0.445	0.515
		PEMCS	0.147	0.707
	AluYb8	Age	0.006	0.942
		Gender	1.417	0.250
		PEMCS	0.121	0.733
	LINE-1	Age	0.327	0.575
		Gender	0.568	0.462
		PEMCS	1.783	0.201

Analyses were done with age as a continuous variable and gender and PEMCS as factors

*Indicates statistical significance, p < 0.05