Fig. 1.

TIGAR upregulation was associated with poor prognosis in AML patients. a Real-time PCR showed that TIGAR mRNA was significantly upregulated in PB cells from three AML patients in comparison with three healthy donors. The TIGAR expression was normalized to 1000 copies of GAPDH expression. b Western blotting analysis showed that the protein expression of TIGAR protein was increased in CD34⁺ BM cells from AML patient versus healthy donor. c Real-time PCR showed that the relative expression of TIGAR mRNA between TIGAR ^low and TIGAR ^high patients. The result represented as mean with S.E. as error bars. d–f Patients from TIGAR ^low group presented significantly longer overall survival (OS) (P = 0.021) (d) and disease-free survival (DFS) (P = 0.028) (e) and lower cumulative incidence of relapse (P = 0.044) (f) than patients from TIGAR ^high group