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. 2016 Nov 25;9:257. doi: 10.1186/s13068-016-0673-0

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Spotting assay of pADH1_FMS1 strains on SCD without pantothenate, containing Δfms1 cells. Cells were grown to exponential phase and adjusted to an OD₆₀₀ of 1. Tenfold serial dilutions were spotted onto SCD lacking pantothenate, whereby 10 µl of Δfms1 overnight culture was added to the medium. Control VSY13 (Δadh1–6 sfa1 gpd2, n-butanol pathway genes, coaA and adhE ^{A267T/E568K/R577S}) and VSY15 (VSY13 with pADH1_FMS1) were compared. Cells were incubated aerobically at 30 °C for 4 days