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. 2016 Nov 25;9:257. doi: 10.1186/s13068-016-0673-0

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — n-Butanol concentrations of n-butanol-producing yeast strains additionally overexpressing enzymes of the n-butanol biosynthesis pathway. Shown are maximum n-butanol titers (^Ca hbd and ^Td ter after 74 h and others after 100 h) of aerobic fermentations in SMD media. Bottleneck analysis was carried out with VSY15 (Δadh1-6 sfa1 gpd2 with n-butanol pathway genes, coaA and adhE ^{A267T/E568K/R577S}, pADH1_FMS1) overexpressing pRS62H (high copy vector, promoter pHXT7, terminator tFBA1) with one each enzyme of n-butanol pathway. As a control, empty vector pRS62H was used. Genes from Sc: Saccharomyces cerevisiae, Ca: Clostridium acetobutylicum, Ec: E.coli, and Td: Treponema denticola are indicated by prefixes in superscript. Error bars represent the standard deviation of three independent replicates