Table 1.
Yeast strains and plasmids used in this study
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| VSY0 |
MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2
adh1::loxP adh3::loxP adh5::loxP adh4Δ::loxP adh2Δ::LEU2 |
[2] |
| Y00000 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 | Euroscarf, Frankfurt |
| Y00595 | BY4741 Mata; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; fms1::kanMX4 | Euroscarf, Frankfurt |
| Y02304 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;
pan6::kanMX4 |
Euroscarf, Frankfurt |
| Y06868 | BY4741 MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;
acs1::kanMX4 |
Euroscarf, Frankfurt |
| JDY2 | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4 | Lab stocks, Boles group |
| VSY4 |
MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; acs1::kanMX4 sfa1Δ: Ec adhE A267T/E568K/hphNT1 |
This work |
| VSY5 |
MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2::kanMX4;
sfa1Δ: Ec adhE A267T/E568K, hphNT1 |
This work |
| VSY7 |
MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec
adhE
A267T/E568K, hphNT1; acs1::kanMX4 |
This work |
| VSY7_evolved | MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec adhE A267T/E568K/R577S, hphNT1; acs1::kanMX4 with unknown mutations for growth on glucose | This work |
| VSY7_R577S |
MATα; his3Δ1; leu2Δ0; lys2Δ0; MET15; ura3Δ0; acs2Δ:Ec
adhE
A267T/E568K/R577S, hphNT1; acs1::kanMX4 |
This work |
| VSY10 |
MATa; ura3-52; trp1-289; leu2-3_112; his3Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP: adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; sfa1Δ: Ec adhE A267T/E568K , hphNT1; adh6Δ: Ec coaA, natNT2; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX |
[2] |
| VSY13 |
MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX |
This work |
| VSY15 |
MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ: Ec coaA, loxP; sfa1Δ: Ec adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX |
This work |
| VSY19 |
MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2
adh1::loxP; adh3::loxP; adh5::loxP; adh4Δ::loxP; adh2Δ::LEU2; adh6Δ:: coaA, loxP; sfa1Δ:: adhE A267T/E568K/R577S , loxP; pFMS1Δ::HIS3, pADH1; ald6Δ; gpd2Δ: Sc ERG10, Ca hbd, Ca crt, Td ter, Ca adhE2, Ec eutE, kanMX |
This work |
| Plasmids | ||
| pRS41H | CEN6ARS4, hphNT1, Ampr | [29] |
| pRS62H | 2µ, hphNT1, Ampr, shortened HXT7 promoter and CYC1
terminator |
[30] |
| pVS4 | CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K | [2] |
| pVS4ev | CEN6ARS4, hphNT1, Ampr, Ec adhE A267T/E568K/R577S | This work |
| pRCC-K_ALD6 | 2µ, kanMX, Ampr, pROX3_Cas9_tCYC1, gRNA for ALD6 | This work |
| pRS62H_ERG10 | 2µ, hphNT1, Ampr, Sc ERG10 | This work |
| pRS62H_hbd | 2µ, hphNT1, Ampr, Ca hbd | This work |
| pRS62H_crt | 2µ, hphNT1, Ampr, Ca crt | This work |
| pRS62H_ter | 2µ, hphNT1, Ampr, Td ter | This work |
| pRS62H_adhE2 | 2µ, hphNT1, Ampr, Ca adhE2 | This work |
| pRS62H_eutE | 2µ, hphNT1, Ampr, Ec eutE | This work |
| pRS41H_pTPI1_NOX | CEN6ARS4, hphNT1, Ampr, NADHoxidase Sp NOX | Lab stocks, Boles group |
Genes from Sc, Saccharomyces cerevisiae; Ca, Clostridium acetobutylicum; Ec, E.coli; Td, Treponema denticola; Sp, Streptococcus pneumoniae are indicated by prefixes in superscript. Promoters are indicated in Additional file 1: Table S2. kanMX G418 resistance, hphNT1 hygromycin resistance, Ampr ampicillin resistance