Fig. 2.

Detection of LRP/LR levels on the surface of pancreatic cancer and neuroblastoma cells. a Quantification of LRP/LR on the surface of pancreatic cancer (AsPC-1) and neuroblastoma (IMR-32) cells. The black peak represents the unlabelled cells, whilst the blue peak represents the cells labelled with goat anti-human phycoerythin (PE)-coupled secondary antibody. The red peak represents the cells labelled with both anti-LRP/LR specific antibody IgG1-iS18 and the afore-mentioned secondary antibody. The inclusion of the unlabelled cells as a control, confirms that the secondary antibody does not bind non-specifically. The shift observed between the black and red peak indicates a change in fluorescence intensity thus the presence of LRP/LR on the cell surface of the cell lines under study. b Determination of chloramphenicol acetyltransferase protein levels on the surface of the three tumorigenic cell lines as a control. The blue peak represents cells that have been labelled with both anti-CAT primary antibody and goat anti-rabbit allophycocyanin (APC)-coupled secondary antibody, whilst the red peak represents the cells labelled with the afore-mentioned secondary antibody only. No distinct shift is seen between the blue and red peak, thereby indicating the absence of the CAT protein on the surface of the cell lines under study