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. 2016 Nov 24;10:526. doi: 10.3389/fnins.2016.00526

Figure 2.

Figure 2

Loss of fractalkine signaling results in increased numbers of RGCs in both early and late stages of decline. (A) Confocal image of retinal flat mount showing Brn3+ nuclei that lack accumulation of somal pNF. (B,C) Confocal image of cells with somal accumulation of pNF, and either expression (B) or absence (C) of Brn3. Orthogonal views in the x-y (bottom) and y-z (side) planes are shown. (D,E) Distribution of the density of cells that are Brn3+/somal pNF+ (D) or Brn3-/somal pNF+ (E) within the central retina in DBA/2J (blue n = 31 retinas) and Cx3cr1gfp/gfp DBA/2J (red n = 36 retinas) mice, sorted in ascending order. Cx3cr1gfp/gfp DBA/2J retinas contain significantly more Brn3+/somal pNF+ cells (D, * p < 0.05; Wilcoxon ranked sum test) and Brn3-/somal pNF+ cells (E, *p < 0.05; Wilcoxon ranked sum test) than DBA/2J retinas. Solid horizontal line indicates the population mean, dashed lines indicate 1 standard deviation above and below the mean. Error bars represent the SEM. Scale bars: 10 μm.