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. 2016 Nov 25;8:274. doi: 10.3389/fnagi.2016.00274

FIGURE 1.

FIGURE 1

Markers of cell proliferation and immature neurons decreased with age in the human SEZ. Cell proliferation (Ki67) and immature neuron (DCX) transcripts were measured by qRT-PCR and normalized to the geometric mean of two housekeeping genes. Ki67 (A) and DCX mRNAs (B) were negatively correlated with age in the SEZ of individuals ranging from 21 to 103 years of age. Ki67 immunohistochemistry was performed in a subset of individuals of the entire cohort ranging from 21 to 81 years of age. Ki67 immunoreactive cells occurred individually, as doublets or small clusters in the lateral wall of the lateral ventricle (C,D). The total number of Ki67 immunoreactive cells in three immunostained sections were counted and normalized to total length of the SEZ. The mean density of Ki67 immunoreactive cells decreased with age; however, this correlation was just at statistical significance (E). Scale bars = 20 μm. LV, lateral ventricle; r, Pearson’s product-moment correlation coefficient; ρ, Spearman’s rank correlation coefficient; sr, semi-partial correlation coefficient.