Component | Volume (μL) | Final amount/concentration |
---|---|---|
DNA + water | 15.7 | 1 ng |
NEB Cutsmart buffer | 1.8 | 1× |
TaqαI (20 U μL−1) | 0.5 | 0.556 U μL−1 |
Total | 18 |
Component | Volume (μL) | Final amount/concentration |
---|---|---|
DNA + water | 15.7 | 1 ng |
NEB Cutsmart buffer | 1.8 | 1× |
TaqαI (20 U μL−1) | 0.5 | 0.556 U μL−1 |
Total | 18 |