| End-prep mix (2 μL per reaction) | Volume (μL) | Final concentration |
|---|---|---|
| Klenow fragment exo-(5 U μL−1) | 1.0 | 0.25 U μL−1 |
| NEB Cutsmart buffer | 0.2 | 1× |
| dNTP mix for RRBS (1 mM dATP, 0.1 mM dCTP, 0.1 mM dGTP) | 0.8 | 40 μM dATP, 4 μM dCTP, 4 μM dGTP (in 20 μL) |
| Total | 2 μL (total reaction volume: 20 μL) |
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