| Component | Volume (μL) |
|---|---|
| Nuclease-free water | 3.5 |
| Bisulfite converted DNA | 19.0 |
| NEBNext Universal primer (10 μM) | 1.25 |
| NEBNext Index primer (10 μM) | 1.25 |
| KAPA HiFi Uracil+ ReadyMix (2×) | 25 |
| Total | 50 |
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