| Step | Temperature (°C) | Time |
|---|---|---|
| 1. Denature | 95 | 2 min |
| 2. Denature | 98 | 20 sec |
| 3. Anneal | 63 | 30 sec |
| 4. Extend | 72 | 1 min |
| Repeat step 2–4 for 5–17 cycles | - | - |
| 5. Extend | 72 | 5 min |
| 6. Hold | 4 | Indefinite |
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