Step | Temperature (°C) | Time |
---|---|---|
1. Denature | 95 | 2 min |
2. Denature | 98 | 20 sec |
3. Anneal | 63 | 30 sec |
4. Extend | 72 | 1 min |
Repeat step 2–4 for 5–17 cycles | - | - |
5. Extend | 72 | 5 min |
6. Hold | 4 | Indefinite |
Step | Temperature (°C) | Time |
---|---|---|
1. Denature | 95 | 2 min |
2. Denature | 98 | 20 sec |
3. Anneal | 63 | 30 sec |
4. Extend | 72 | 1 min |
Repeat step 2–4 for 5–17 cycles | - | - |
5. Extend | 72 | 5 min |
6. Hold | 4 | Indefinite |