Component | Volume (μL) |
---|---|
End-repaired/dA-tailed DNA from Step 6A(viii) | 65 |
Blunt/TA ligase mix | 15 |
Ligation enhancer | 1 |
Methylated adaptors (15 μM) | 2.5 |
Total | 83.5 |
Component | Volume (μL) |
---|---|
End-repaired/dA-tailed DNA from Step 6A(viii) | 65 |
Blunt/TA ligase mix | 15 |
Ligation enhancer | 1 |
Methylated adaptors (15 μM) | 2.5 |
Total | 83.5 |